In vitro fertilisation is fertilisation of eggs outside the body . It was carried out successfully in humans in 1977 and now is a routine method of fertility treatment with thousands of IVF centres all over India.
IVF consists of following steps :
Ovarian stimulation:– The woman has her ovaries stimulated using hormones which are daily injections to be administered in the fat. These hormones help her grow multiple eggs rather than one single egg as usually happens in a natural cycle. A trigger injection which helps in maturing eggs is administered after 10 to 12 days of hormones.36 hours later eggs are collected from the woman’s body.
Egg retrieval :- eggs are retrieved under GA and are placed in special culture medium in an incubator in the lab.
Sperm collection :- The Male partner is asked to ejaculate and collect semen sample which is prepared so that healthiest sperm are used for IVF.
Insemination :- Sperm and eggs are incubated overnight and the eggs are checked next day for fertilisation.
Embryo development :- fertilised egg develop into embryos and are grown in incubator upto 6 days.
Embryo Transfer :- The best embryos are selected and are placed in the uterus using thin plastic tubes. This is quick , simple and painless procedure performed without sedation.
Embryo Freezing :- Any spare good quality embryos can be frozen for further cycles. All the embryos can be frozen too using next cycle for embryo transfer as statistically frozen embryo transfers give better results as the females body has come out of the effect of massive dose of hormones used for stimulation. Cryopreservation incurs extra charge , charged by the IVF lab.
Pregnancy test – approximately 2 weeks later pregnancy test is performed.
Sometimes donor egg or sperm might be needed based on the specific problem of the couple.